Promega 100 Bp Ladder Manual

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PCR conditions were as follows: 20 μL of 5 × PCR buffer (GoTaq-Promega), 5 Thus, a 100 bp DNA ladder with a range outside of 1000 bp could be generated.

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Page 3 of 3 Labeling Protocols The 100 bp DNA Ladder can be radioactively labeled by T4 DNA polymerase or T4 polynucleotide kinase. T4 DNA polymerase is recommended

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The 100 bp DNA Ladder is a set of 10 easy-to-remember band sizes for use in qualitative agarose gel analysis. The ladder is supplied in ready-to-load form in gel

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